Cycling an Aquarium

chvvkumar

Active Member
View Badges
Joined
Feb 4, 2019
Messages
123
Reaction score
93
Location
St. Louis
Rating - 0%
0   0   0
Set up my first FOWLR tank and I am looking for help in interpreting my results.

I have dry rock and live sand and no fish or corals or inverts in my tank.

I added Bio spira and ammonia (1 drop per gallon) four days ago. I did a test today (was waiting for my test kit to arrive and it did today):

IMG_20200505_145840.jpg


IMG_20200505_150010.jpg


Am I correct that the parameters are as follows:
pH: 8.0
Ammonia: 0.5ppm
Nitrite: 0.5ppm
Nitrate: 20ppm
Does this mean the cycle has stalled?
 

brandon429

why did you put a reef in that
View Badges
Joined
Dec 9, 2014
Messages
30,220
Reaction score
24,063
Location
tejas
Rating - 0%
0   0   0
opinions vary on procedure still just a little, and thats good as it evolves procedures and efficiency im all for it. my take is this: i have on file a biospira skip cycle thread where fish were added with dry rock on day 1.

so this tank is that tank, no fish, + 3 more days, plus wet sand inoculant. I dont factor api test kits, I factor work thread patterns to make most calls. if a fish is added here it will live to the degree Ive never seen a report of biospira confirmed as a dead bottle. there is an exacting way to test it using the current tools above, and if reconfirmation is wanted then add ammonia until that test results gets slightly darker than above, not much, slightly.

wait 24 hours then retest, if back down, confirmed. my cycle calls are only based on ammonia control, effectively eliminating 2x mistesting possibilities from the assessment. we uphold that single param assessment as long as things keep not dying, and on the seneye kits keep showing .00X ppm which is the accepted safe zone free ammonia conversion rate per todays best tests. I think a seneye would show thousandths ppm above and a fish added would not die.
 
Last edited:

chvvkumar

Active Member
View Badges
Joined
Feb 4, 2019
Messages
123
Reaction score
93
Location
St. Louis
Rating - 0%
0   0   0
opinions vary on procedure still just a little, and thats good as it evolves procedures and efficiency im all for it. my take is this: i have on file a biospira skip cycle thread where fish were added with dry rock on day 1.

so this tank is that tank, no fish, + 3 more days, plus wet sand inoculant. I dont factor api test kits, I factor work thread patterns to make most calls. if a fish is added here it will live to the degree Ive never seen a report of biospira confirmed as a dead bottle. there is an exacting way to test it using the current tools above, and if reconfirmation is wanted then add ammonia until that test results gets slightly darker than above, not much, slightly.

wait 24 hours then retest, if back down, confirmed. my cycle calls are only based on ammonia control, effectively eliminating 2x mistesting possibilities from the assessment. we uphold that single param assessment as long as things keep not dying, and on the seneye kits keep showing .00X ppm which is the accepted safe zone free ammonia conversion rate per todays best tests. I think a seneye would show thousandths ppm above and a fish added would not die.

Thank you Brandon, I will re-test and see what the trend looks like. Appreciate your time :)
 
OP
OP
Brew12

Brew12

Electrical Gru
View Badges
Joined
Aug 14, 2016
Messages
22,488
Reaction score
61,061
Location
Decatur, AL
Rating - 0%
0   0   0
Set up my first FOWLR tank and I am looking for help in interpreting my results.

I have dry rock and live sand and no fish or corals or inverts in my tank.

I added Bio spira and ammonia (1 drop per gallon) four days ago. I did a test today (was waiting for my test kit to arrive and it did today):

IMG_20200505_145840.jpg


IMG_20200505_150010.jpg


Am I correct that the parameters are as follows:
pH: 8.0
Ammonia: 0.5ppm
Nitrite: 0.5ppm
Nitrate: 20ppm
Does this mean the cycle has stalled?
Most people wouldn't considered a cycle stalled until it had gone a week or more without changing. Since you don't know where you started it is hard to say where you should be.

In most tanks it would take some serious overfeeding to get ammonia to 1ppm in a day. There are many ways to get a tank ready for fish. You have started going down the path of using a bottled bacteria while verifying it works by adding ammonia. Since you went down this path, you added an unnatural amount of ammonia to your system. I would recommend being patient and waiting for that nitrite to drop. If you want to dose ammonia back up again after that happens is up to you.
 

chvvkumar

Active Member
View Badges
Joined
Feb 4, 2019
Messages
123
Reaction score
93
Location
St. Louis
Rating - 0%
0   0   0
Most people wouldn't considered a cycle stalled until it had gone a week or more without changing. Since you don't know where you started it is hard to say where you should be.

In most tanks it would take some serious overfeeding to get ammonia to 1ppm in a day. There are many ways to get a tank ready for fish. You have started going down the path of using a bottled bacteria while verifying it works by adding ammonia. Since you went down this path, you added an unnatural amount of ammonia to your system. I would recommend being patient and waiting for that nitrite to drop. If you want to dose ammonia back up again after that happens is up to you.
Thank you for your time, I will wait and see if ammonia drops and other parameters follow this graph before doing anything:

1588716863575.png
 

canadianeh

Valuable Member
View Badges
Joined
Feb 28, 2017
Messages
1,611
Reaction score
1,044
Rating - 0%
0   0   0
Regarding CUC, how do I know when my tank needs them?

Also I appreciate it if you can give me your opinion on my cycling process right now.

Cycling using Dr Tim’s products and I am also using Caribsea purple liferock

Day 1
Dose 60 drops of ammonia

Day 2
Ammonia <0.15
Nitrate 25

Day 3
Ammonia <0.15
Nitrate 2.5
Then after added more ammonia drops

Day 4
Ammonia 0.25
Nitrite 0
Nitrate 2.5

Day 5
Ammonia 0.25
Nitrite 0
Nitrate 2

Day 6
Added more ammonia
15 minutes later ammonia test shows 0.5

Day 7
Ammonia 0.5
Nitrite 0.5
Nitrate 5

According to dr Tim’s, on day 9 I can add fish.

@Brew12 so on day 8 didn’t test and didn’t dose ammonia.

Day 9 today I did a test and didn’t does ammonia and the result is weird particularly the ammonia. It stays the same from day 7

Ammonia 0.5
Nitrite 0.25
Nitrate 25 ppm

Is this normal?
 
OP
OP
Brew12

Brew12

Electrical Gru
View Badges
Joined
Aug 14, 2016
Messages
22,488
Reaction score
61,061
Location
Decatur, AL
Rating - 0%
0   0   0
@Brew12 so on day 8 didn’t test and didn’t dose ammonia.

Day 9 today I did a test and didn’t does ammonia and the result is weird particularly the ammonia. It stays the same from day 7

Ammonia 0.5
Nitrite 0.25
Nitrate 25 ppm

Is this normal?
Unfortunately, ammonia tests often provide false readings. I would give it another day or two. That may be how your system/test kit reads zero.
 

canadianeh

Valuable Member
View Badges
Joined
Feb 28, 2017
Messages
1,611
Reaction score
1,044
Rating - 0%
0   0   0
Unfortunately, ammonia tests often provide false readings. I would give it another day or two. That may be how your system/test kit reads zero.
Day 10 was yesterday, and I did another test. Ammonia and Nitrate same result as day 9 and Nitrite went up to 0.5
I dosed ammonia again yesterday after the test. Should I just still leave it and continue to dose ammonia every 3 days?

can I add Brightwell Microbacter XLM now to add more beneficial bacteria? I used Dr Tims from day 1.

Day 1
Dose 60 drops of ammonia

Day 2
Ammonia <0.15
Nitrate 25

Day 3
Ammonia <0.15
Nitrate 2.5
Then after added more ammonia drops

Day 4
Ammonia 0.25
Nitrite 0
Nitrate 2.5

Day 5
Ammonia 0.25
Nitrite 0
Nitrate 2

Day 6
Added more ammonia
15 minutes later ammonia test shows 0.5

Day 7
Ammonia 0.5
Nitrite 0.5
Nitrate 5

Day 8
Didn’t test. No ammonia added

Day 9
Ammonia 0.5
Nitrite 0.25
Nitrate 25

Day 10
Ammonia 0.5
Nitrite 0.5
Nitrate 25
Added 65 drops of ammonia after the test
 
Last edited:

Darsh

Active Member
View Badges
Joined
Nov 4, 2018
Messages
497
Reaction score
451
Location
Poland
Rating - 0%
0   0   0
I'm started my 100 gallon reef tank 5 weeks ago on 90lbs dry rock and dry sand, adding 70ml 10% ammonia solution and 16oz. Microbe-lift SPECIAL BLEND bacteria to start cycling.
My biological filtration is 1kg MAXSPECT BioSpheres in sump. After 5 weeks NH3 = 0 / NO2=0 / and my NO3 is maxout on Salifert test scale >100ppm.
Today i addet some Chaetomorpha to sump and start lightning it for 12h.
How can i reduce NO3?
Should I wait?
Wat is the NO3 safe level to clown fish?
50gallon water cheange is the last thing I would like to do. (time to produce water and buying new salt)
If your Chaeto is growing, it should lower your Nitrates.
50% water change will bring your Nitrates down to 50ppm, which will be okay for clownfish but I prefer <25ppm and keep mine at <10ppm.
To lower them another way, check out this thread:
 
Last edited:

canadianeh

Valuable Member
View Badges
Joined
Feb 28, 2017
Messages
1,611
Reaction score
1,044
Rating - 0%
0   0   0
@Brew12 Day 14 and The nitrite and nitrate numbers finally moved. Ammonia still shows the same for some reason. I just bought a fresh dr Tim’s ammonia. I want to try to dose using the fresh one. Should I do 20% WC first to export the nitrate and then dose ammonia again? Or can I dose first then WC later?

Day 1
Dose 60 drops of ammonia

Day 2
Ammonia <0.15
Nitrate 25

Day 3
Ammonia <0.15
Nitrate 2.5
Then after added more ammonia drops

Day 4
Ammonia 0.25
Nitrite 0
Nitrate 2.5

Day 5
Ammonia 0.25
Nitrite 0
Nitrate 2

Day 6
Added more ammonia
15 minutes later ammonia test shows 0.5

Day 7
Ammonia 0.5
Nitrite 0.5
Nitrate 5

Day 8
Didn’t test. No ammonia added

Day 9
Ammonia 0.5
Nitrite 0.25
Nitrate 25

Day 10
Ammonia 0.5
Nitrite 0.5
Nitrate 25
Added 65 drops of ammonia after the test

Day 11
Ammonia 0.5
Nitrite 0.5
Nitrate 50

Day 12
Ammonia 0.25
Nitrite 1
Nitrate 50

Day 13
Ammonia 0.25
Nitrite 1
Nitrate 50

Day 14
Ammonia 0.25
Nitrite 2
Nitrates 100
 
OP
OP
Brew12

Brew12

Electrical Gru
View Badges
Joined
Aug 14, 2016
Messages
22,488
Reaction score
61,061
Location
Decatur, AL
Rating - 0%
0   0   0
@Brew12 Day 14 and The nitrite and nitrate numbers finally moved. Ammonia still shows the same for some reason. I just bought a fresh dr Tim’s ammonia. I want to try to dose using the fresh one. Should I do 20% WC first to export the nitrate and then dose ammonia again? Or can I dose first then WC later?

Day 1
Dose 60 drops of ammonia

Day 2
Ammonia <0.15
Nitrate 25

Day 3
Ammonia <0.15
Nitrate 2.5
Then after added more ammonia drops

Day 4
Ammonia 0.25
Nitrite 0
Nitrate 2.5

Day 5
Ammonia 0.25
Nitrite 0
Nitrate 2

Day 6
Added more ammonia
15 minutes later ammonia test shows 0.5

Day 7
Ammonia 0.5
Nitrite 0.5
Nitrate 5

Day 8
Didn’t test. No ammonia added

Day 9
Ammonia 0.5
Nitrite 0.25
Nitrate 25

Day 10
Ammonia 0.5
Nitrite 0.5
Nitrate 25
Added 65 drops of ammonia after the test

Day 11
Ammonia 0.5
Nitrite 0.5
Nitrate 50

Day 12
Ammonia 0.25
Nitrite 1
Nitrate 50

Day 13
Ammonia 0.25
Nitrite 1
Nitrate 50

Day 14
Ammonia 0.25
Nitrite 2
Nitrates 100
You can't trust your nitrate number until your nitrite reads 0. I would dose first and then do a water change, if needed, when nitrite is 0ppm.
 

canadianeh

Valuable Member
View Badges
Joined
Feb 28, 2017
Messages
1,611
Reaction score
1,044
Rating - 0%
0   0   0
You can't trust your nitrate number until your nitrite reads 0. I would dose first and then do a water change, if needed, when nitrite is 0ppm.

Oh I must have misunderstood then. I thought there is no beneficial bacteria that can consume Nitrate and only algae such as chaeto or WC to deal with Nitrate?
 
OP
OP
Brew12

Brew12

Electrical Gru
View Badges
Joined
Aug 14, 2016
Messages
22,488
Reaction score
61,061
Location
Decatur, AL
Rating - 0%
0   0   0
Oh I must have misunderstood then. I thought there is no beneficial bacteria that can consume Nitrate and only algae such as chaeto or WC to deal with Nitrate?
You are partially correct. There is an anaerobic bacteria that can break nitrate down to nitrogen gas, but it only happens deep in live rock or in special plates. But.. nitrate tests work by breaking nitrate down to nitrite and then measuring it. So, your nitrate test is actually a combination of nitrate and nitrite, and a small amount of nitrite will cause a much larger nitrate reading.
 

canadianeh

Valuable Member
View Badges
Joined
Feb 28, 2017
Messages
1,611
Reaction score
1,044
Rating - 0%
0   0   0
You are partially correct. There is an anaerobic bacteria that can break nitrate down to nitrogen gas, but it only happens deep in live rock or in special plates. But.. nitrate tests work by breaking nitrate down to nitrite and then measuring it. So, your nitrate test is actually a combination of nitrate and nitrite, and a small amount of nitrite will cause a much larger nitrate reading.

I got my ammonia up to 1.5 ppm. I couldn’t get it this high with my old ammonia. So it has to be my old ammonia. It went bad.

I see. My sandbed is 1” deep only. But in addition to the Caribsea liferock I also have bunch of marinepure bio balls. Is there a way to test if I have this anaerobic bacteria? Or I just have to see if my nitrate can go down without water change?
 
OP
OP
Brew12

Brew12

Electrical Gru
View Badges
Joined
Aug 14, 2016
Messages
22,488
Reaction score
61,061
Location
Decatur, AL
Rating - 0%
0   0   0
I got my ammonia up to 1.5 ppm. I couldn’t get it this high with my old ammonia. So it has to be my old ammonia. It went bad.

I see. My sandbed is 1” deep only. But in addition to the Caribsea liferock I also have bunch of marinepure bio balls. Is there a way to test if I have this anaerobic bacteria? Or I just have to see if my nitrate can go down without water change?
I'm not aware of a way to test it, but it's normally a very minor way to reduce nitrates anyway. I don't know of anyone that uses it as a major way to export nitrate.
 

Lewism11

Community Member
View Badges
Joined
May 11, 2020
Messages
39
Reaction score
66
Rating - 0%
0   0   0
I never measured any nitrite during my cycle, is this a problem?

No, odds are this is not a problem. It is possible that you had a larger initial population of Nitrobacter bacteria (nitrite eaters) compared to Nitrosomonas bacteria (ammonia eaters). In this case the nitrite was processed to nitrate almost as soon as it was produced.

Another possibility is that you have a large population of Nitrospira bacteria. These bacteria convert ammonia into nitrite and then to nitrate within the same cell structure so the nitrite is never released into the water to be measured.


Hey, been out of the reef tank hobby for a few years and now I'm trying to get back into it. Would the above be true if I am ready .50 ppm for Ammonia and 10 ppm for Nitrates but 0 for Nitrites, it's been running for about a week. I'm seeing a slow increase in Nitrates (started at 0 ppm) and over the last week it has climbed to 10 ppm. Ammonia has been holding steady at .50 ppm since day 3. I started this tank with 75% dry rock and 25% live rock from my LFS sump.
 
OP
OP
Brew12

Brew12

Electrical Gru
View Badges
Joined
Aug 14, 2016
Messages
22,488
Reaction score
61,061
Location
Decatur, AL
Rating - 0%
0   0   0
I never measured any nitrite during my cycle, is this a problem?

No, odds are this is not a problem. It is possible that you had a larger initial population of Nitrobacter bacteria (nitrite eaters) compared to Nitrosomonas bacteria (ammonia eaters). In this case the nitrite was processed to nitrate almost as soon as it was produced.

Another possibility is that you have a large population of Nitrospira bacteria. These bacteria convert ammonia into nitrite and then to nitrate within the same cell structure so the nitrite is never released into the water to be measured.


Hey, been out of the reef tank hobby for a few years and now I'm trying to get back into it. Would the above be true if I am ready .50 ppm for Ammonia and 10 ppm for Nitrates but 0 for Nitrites, it's been running for about a week. I'm seeing a slow increase in Nitrates (started at 0 ppm) and over the last week it has climbed to 10 ppm. Ammonia has been holding steady at .50 ppm since day 3. I started this tank with 75% dry rock and 25% live rock from my LFS sump.
What you are seeing is pretty normal for using live rock. I'm not sure what you are using for an ammonia source but if it is something in constant decay (like fish food or a shrimp) what you are seeing is exactly what you want.
 

Lewism11

Community Member
View Badges
Joined
May 11, 2020
Messages
39
Reaction score
66
Rating - 0%
0   0   0
I haven't added anything for an ammonia source, in my previous tanks I simply added live rock and let the cycle do its thing.
 

HAVE YOU EVER KEPT A RARE/UNCOMMON FISH, CORAL, OR INVERT? SHOW IT OFF IN THE THREAD!

  • Yes!

    Votes: 32 45.7%
  • Not yet, but I have one that I want to buy in mind!

    Votes: 9 12.9%
  • No.

    Votes: 26 37.1%
  • Other (please explain).

    Votes: 3 4.3%
Back
Top