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Very good computationOn the other hand....
3 frozen cubes, (3g each) = roughly 10g.
Estimate 90% water weight and pretend the rest is protein.
1g protein = 0.16g (160mg) Nitrogen
In 225 gallons (900L) gives 160mg/900L =
0.18mg/L N.
So we're talking ~0.2ppm total ammonia per day. After heterotrophs get done eating their fill, you might not even detect that on a test kit.
Many we see with food will :