Our tank's biology up close

[jason2459]

Nothing at all to do with the above strand that the bacteria are attached to but very cool

Bacteria nano wires
Http://phys.org/news/2014-08-bacterial-nanowires-thought.amp

"Shewanella bacteria roughly 1/50th the width of a human hair reach out nanowires to breathe when deprived of oxygen. These types of bacteria are typically found underground or in lake or ocean sediments, where oxygen is scarce. Credit: PNAS, DOI: 10.1073/pnas.1410551111"

http://m.pnas.org/content/111/35/12883


And more cool research on bacteria immune system that just got published yesterday
http://m.phys.org/news/2016-11-bacteria-ramp-immune-response-viral.html

 

[jason2459]

I did get a closer look tonight at Vibrant. And I keep wondering if I've done something to cause those stands to appear. I'm starting to think not. Especially since I haven't seen them in any other samples I've ever done with bacteria in a bottle, in tank's samples, or many other samples I've done with my son from around the house, body, and yard.

Uploading pics and vids now. To make sure it wasn't how I prepared (cleaned/sterilized) the slides I looked at a sample completely unprepared. Thinking maybe it's the cover slip somehow I viewed another sample with out the coverslip and in a well slide. Then used a little stain and rinsing. Etc.

Found strands in every instance. In some cases I found lots as if I smeared the sample and stain which I didn't.

Then the last sample I looked at I found the mother of all lines...

No idea what it is. It doesn't stain with violet or iodine or safranin. But that last "line" was very interesting and had a few even thinner stands starting off it.


Edit :
it's not entirely dormant. There was some active movement.

 

[jason2459]

Look a shiny object !

OMG, this is so awesome!!!!

http://www.nikonsmallworld.com/subjects/main/zebrafish

 

[jason2459]

Ok so this was another look at Vibrant a bit more upclose.

Vibrant_part2-0001 by Jason, on Flickr

Vibrant_part2-0003 by Jason, on Flickr

Vibrant_part2-0045 by Jason, on Flickr

Vibrant_part2-0046 by Jason, on Flickr

Vibrant_part2-0047 by Jason, on Flickr

Vibrant_part2-0048 by Jason, on Flickr

Vibrant_part3-0051 by Jason, on Flickr

 

[jason2459]

Another sample with out cleaning the slide thinking maybe I'm doing something in the cleaning process causing streaks and the bacteria is "clinging" to those streaks.

Nope, still there
Vibrant_part2-0027 by Jason, on Flickr

Vibrant_part2-0029 by Jason, on Flickr

 

[jason2459]

So, now for some stain. Just did a basic gram stain process: cleaned the slide, added the sample, burned it onto the slide, added some violet, rodi rinsed, added some iodine, rodi rinsed, light ethyl alcohol rinse, rodi rinse, add some safranin, final rodi rinse, and dry.

And, after all that, still there. But in this third sample for the night I found a monster of a strand.

Vibrant_part3-0051 by Jason, on Flickr

Vibrant_part3-0052 by Jason, on Flickr

Vibrant_part3-0053 by Jason, on Flickr

Vibrant_part3-0054 by Jason, on Flickr

Vibrant_part3-0056 by Jason, on Flickr

Vibrant_part3-0057 by Jason, on Flickr

Vibrant_part3-0060 by Jason, on Flickr

Vibrant_part3-0061 by Jason, on Flickr

Vibrant_part3-0065 by Jason, on Flickr

Vibrant_part3-0066 by Jason, on Flickr

 

[jason2459]

Vibrant_part3-0067 by Jason, on Flickr

Vibrant_part3-0068 by Jason, on Flickr

Vibrant_part3-0069 by Jason, on Flickr

Vibrant_part3-0070 by Jason, on Flickr

Vibrant_part3-0071 by Jason, on Flickr

Vibrant_part3-0072 by Jason, on Flickr

Vibrant_part3-0073 by Jason, on Flickr

Vibrant_part3-0074 by Jason, on Flickr

 

[jason2459]

And last night I took a look at the 3rd sample I've been wanting to get to and found something that now makes me wonder if the strands above in the Vibrant bottle aren't algae based. I wasn't think it was algae based at all as they are thin to extremely thing strands.

BUT I found this (more photo's from last night to come)

Some kind of strand seen in the algae
Hair_Algae_Pump-0070 by Jason, on Flickr

Chains of bacteria coming off it
Hair_Algae_Pump-0071 by Jason, on Flickr

 

[jason2459]

Video playlist of the Vibrant samples. There was some active live bacteria in there as well.

https://www.youtube.com/playlist?list=PLN_wI2B-a8vrHDF6tr1G4MWUYrW3ZbFrc

 

[UWC]

If they were more uniformed shaped, we'd call them wipers

 

[jason2459]

The purple does confirm what was already suspected of the gram positive nature of streptococcus.

Vibrant_part3-0056 by Jason, on Flickr

 

[jason2459]

If they were more uniformed shaped, we'd call them wipers

Can you confirm that those strands are not something I'm doing to contaminate the samples? Or even confirm what those strands are that bacteria are clinging to? Email is fine too?

 

[UWC]

Can you confirm that those strands are not something I'm doing to contaminate the samples? Or even confirm what those strands are that bacteria are clinging to? Email is fine too?

You are doing just fine

 

[jason2459]

If they were more uniformed shaped, we'd call them wipers

Are you refering to something like a microfiber cleaning wiper?

 

[UWC]

Windshield wipers

 

[jason2459]

Windshield wipers

lol ok

This is what I was thinking

https://books.google.com/books?id=z...QAhUILmMKHdQqADAQ6AEIHzAB#v=onepage&q&f=false

 

[Elementalj]

lol ok

This is what I was thinking

https://books.google.com/books?id=z...QAhUILmMKHdQqADAQ6AEIHzAB#v=onepage&q&f=false

Boy, you don't screw around! [emoji122][emoji122][emoji122]

 

[UWC]

lol ok

This is what I was thinking

https://books.google.com/books?id=z...QAhUILmMKHdQqADAQ6AEIHzAB#v=onepage&q&f=false

Not trying to confuse you. Just don't want to say too much

 

[jason2459]

Not trying to confuse you. Just don't want to say too much

Totally understood. [emoji4]

 

[Rick.45cal]

Pretty clever UWC