I don't think the bacteria is necessarily going to colonize the filter. There is no ammonia for them to process if you use fresh saltwater. Some may get trapped in the media I guess.I'd suggest getting a bowl or Tupperware and soaking the Tim's directly into the media. If it isn't completely submerged, then top it off with FRESH SALTWATER. This is EXACTLY what I was told to do. My uneducated guess is it forces the bacteria to colonise the media. I had to setup 4 qts after ttm(and two wrasses I have coming next week) and all qts are reading 0 ammonia a week later