Dinoflagellates – Are You Tired Of Battling Altogether?

thrillreefer

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Brown, stringy growths that diminish overnight. Ostreopsis ovata? 200x magnification. They also seem to be throwing off clear "husks" which is unexpected. What should I do? Have UV running; have been dosing phosphate (measures at zero usually).
20240304_Dinoflag_Slide_D_p01_0_A01f00d3 copy.jpg
 

tetonair

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I don't know what percentage of folks had luck battling dinos with any of the methods in the old Dino thread but it's obviously a very low percentage, so I'd like refresh folks on the natural alternatives and lay out three areas of info:
  • some of the factors that contribute to a dino outbreak
  • how to avoid common dino outbreaks
  • and what do if your tank is already having an outbreak
Let's get started!

Common Contributing Factors
Some of the most common factors that contribute to the dino outbreaks we cover in this thread are:
  • the tank being new, rock being immature or the tank being otherwise highly disturbed, such as by other harsh tank treatments
  • hard core nutrient reduction tools being used, such as
    • organic carbon dosing
    • excess "bio media"
    • algae filtration
    • nutrient adsorbing media like GFO
These four factors, or excess nutrient removal generally, play – usually in combination; rarely just one factor alone – pretty directly into dino's conversion to the blooming, phagotrophic, mat forming, toxin-producing side of their nature.
  • Starvation Is Their Cue
    • Dino's seem to prefer life as autotrophic epiphytes on macro algae – chaeto morpha seems to be one of their favorite types to host in. (Maybe this fact can be useful to us; maybe sometimes chaeto ought not be used, or used with special consideration)
    • For several reasons, dino's seem to be terrible at nutrient uptake. This means they are more prone to starvation than many or most other microorganisms they have to compete with.....especially bacteria, which can scavenge free nutrients down to CRAZY low levels...low enough to starve out other microbes or algae.
    • With their protective mucus mats, potential to generate wicked toxins, and ability to survive not only by way of photosynthesis and dissolved nutrients, but alternately, when times get tough, by "eating their neighbors". (The least of their tricks.)
    • Dino's generally gain a competitive edge against their competitors AND their predators in a nutrient-starved environment. Keep reading!
How To Avoid Having A Dino Outbreak
In a nutshell, here's how to avoid dino outbreaks and begin to normalize your tank if you already have an outbreak:
  • Phosphate Control
  • Nitrate Control
  • Starvation conditions (zero or near-zero nitrate or phosphate levels) should be avoided.
    • Keep in mind that dissolved nutrients are not "waste products" to be eliminated
    • They are nutrients for the critters you care about like corals
    • The are also nutrients for a potential multitude of mostly-unknown/anonymous microbes that are needed to bring stability to a new tank.
    • Once excess nutrients have an impact, in fact, they usually can't be simply eliminated with media anyway – they've probably already had an impact on the tank's microbial cycle. (See blog link #3 at bottom.)
    • This all adds up to skipping almost all "extra" nutrient removing steps during the tank's initial development. This period seems to be especially critical, and longer in a tank started with dry, dead rock. Don't use anything until it's absolutely needed and other options have been fully exhausted...and be conservative with how you apply any nutrient removing tool.
What to do if you're tank is already having a dino outbreak
When attempting to control an organism like a dinoflagellate, confirming the ID will help, if possible:
  • So to begin with, make sure you have Dinos – you should have multiple factors at work...these factors were mentioned in the first section above. The less these factors seem to describe your tank, the less likely any of this advice will be correct for your situation – so post questions! :)
    • no special equipment is needed to confirm whether your algae sample has dino's and/or other algae
    • Use @taricha's dino confirmation guide on posts #986-987.
  • Once you have confirmed that you have dino's you should ideally figure out what type(s) your tank is hosting. (Multiple species blooms seem almost as common as single-strain blooms.)
    • A basic 1200x microscope will be useful and doesn't have to be fancier than a $15 toy scope. Even a $50 scope is a lot nicer, if you think you might be more serious about it.
    • See: Selecting a microscope for more discussion.


  • Extra Measures
    Generally, these tools will give extra control in terms of removing and/or killing cells in the water column....usually, along with other measures explained here, expediting the close of the dino bloom.
    • UV
      You can find discussions throughout the thread by using this search, with a great breakout of spec's on post #3770.
    • Diatom Filtration
      Effective, but not that popular. The more common units like the classic Vortex are somewhat difficult to use, and the newer units like the new Marineland Polishing Filter are relatively unknown. Still worthy of consideration.
So, after you get a measure of control, make sure you read What is the End Game?

Miscellaneous Goodies

  • Take measures to assure that your feeding system is very consistent. An auto-feeder is an overlooked tool on most tanks. Look at Eheim's feeders...set them on low with high quality flake food. Just don't let them run your whole feeding program as flake isn't great food.

  • Find out what inconsistencies you can eliminate with your husbandry to prevent more unneeded disturbances and the resulting microbial/algal changes. This could be changes to lighting or water chemistry – make them as consistent as you can.

  • E.g. If you're adding new livestock all the time, stop it. If you have a color-tunable light fixture, stop re-tuning the colors. If you don't have an ATO keeping your salinity stable, get one. If you're still managing your dosing by hand, get an $80 4-head doser. Etc.

  • If you provide the stability, then your dino's competitors will start competing with them and their predators will start eating them!!

  • One thing that seems to help things progress is to stop scraping down the algae off your glass....once the dino's start giving up space that is. Mechanical removal is a legit short-term strategy and might help give competitors a leg up too.



Other interesting more-or-less related links on my blog:
(Also cross-posted in the old Dino thread!)
I really like this post, seems like even more specific information than what can be found over on Mac’s Facebook page.
Here is my video. Is this LCA or SCA?
Tank looks good, 3months old, uglies have subsided.
P04 .05 // N03 3.1
Just got the microscope.
I am only finding 5/slide and no Diatom.
Should I be worried?
 

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ScottB

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Brown, stringy growths that diminish overnight. Ostreopsis ovata? 200x magnification. They also seem to be throwing off clear "husks" which is unexpected. What should I do? Have UV running; have been dosing phosphate (measures at zero usually).
20240304_Dinoflag_Slide_D_p01_0_A01f00d3 copy.jpg
Yes, ostreopsis. UV generally will knocks these back. But it needs to be 1 watt / 3 gallons, with a newish bulb. Run the pump slow to increase cook time. Ignore manufacturer's sizing recommendations.
 

ScottB

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I really like this post, seems like even more specific information than what can be found over on Mac’s Facebook page.
Here is my video. Is this LCA or SCA?
Tank looks good, 3months old, uglies have subsided.
P04 .05 // N03 3.1
Just got the microscope.
I am only finding 5/slide and no Diatom.
Should I be worried?
LCA. The hooked beak is the tell.

Honestly, LCA are not really a big deal. Not very toxic at all and generally remain in the sand. You can bump up the diatom population with some silicate dosing if you like.
 

thrillreefer

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@ScottB will dosing silicate to increase diatoms help with Ostreopsis? On top of upsizing my UV. (Currently running 9 watt green killing machine in 50 gal water, and fairly old.)
 

ScottB

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@ScottB will dosing silicate to increase diatoms help with Ostreopsis? On top of upsizing my UV. (Currently running 9 watt green killing machine in 50 gal water, and fairly old.)
Aiding any other surface competitors is going to be a good thing, but a well functioning and properly sized UV is the more effective tool for ostreos.
 

thrillreefer

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Sounds good. I also have Bryopsis starting to get out of control since I was dosing phosphates to fight the Dinos… will hit that with fluconazole, and buy a real UV to be ready after that treatment completes. Or if needed sooner. Eyeing the Aqua UV advantage 2000 15w + Sicce 1.5.
 

ScottB

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Sounds good. I also have Bryopsis starting to get out of control since I was dosing phosphates to fight the Dinos… will hit that with fluconazole, and buy a real UV to be ready after that treatment completes. Or if needed sooner. Eyeing the Aqua UV advantage 2000 15w + Sicce 1.5.
Aqua UV makes good units and that should get it done for ostreos.
 

Mickey

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I've been fighting dinos plus cyano off and on for several years. Take is old and established since 2004. Nutrients have always run high. Phosphates over 1.00 (decimal in the right place). For the last 6-9 months some major problems. Several corals dying slow deaths. Euphelia's have closed up for weeks. and Large Duncan coral closed up and totally withdran for three months. All zoos closed and fading - mostly spread throughout my reef from a colony added many years ago, all gone. Lots of palythoas on back wall of overflow that I've wanted to get rid of for years with little luck, but now mostly all closed up and shrinking away (that's the only good thing).

Been dosing silica for almost two years to keep level between .5 and 1.0. diatoms are plentiful and almost a problem. Cyano is exclusively on the rocks. Looking under microscope the left side of tank is mostly cyano which hydrogen peroxide show impact, right side of tank is mostly spirolina which Chemiclean should impact. Hesitant to use Chemiclean as I'm afraid that's part of what drove things to this point since I used it previously. Tried H2O2 treatment for several weeks for the cyano but saw no impact.

I am thinking about a UV but it's cost prohibitive with 240 gallon system. At recommended 1 watt per 3 gallons thats an 80 watt unit which is pretty hefty price. I've also tried blackouts with no impact. I know it sounds like I've messed with things a lot but for the most part I've left things alone hoping it will right itself and that's why I've been fighting it for so long.

Any suggestions? I really don't want to tear things down.
 

ScottB

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I've been fighting dinos plus cyano off and on for several years. Take is old and established since 2004. Nutrients have always run high. Phosphates over 1.00 (decimal in the right place). For the last 6-9 months some major problems. Several corals dying slow deaths. Euphelia's have closed up for weeks. and Large Duncan coral closed up and totally withdran for three months. All zoos closed and fading - mostly spread throughout my reef from a colony added many years ago, all gone. Lots of palythoas on back wall of overflow that I've wanted to get rid of for years with little luck, but now mostly all closed up and shrinking away (that's the only good thing).

Been dosing silica for almost two years to keep level between .5 and 1.0. diatoms are plentiful and almost a problem. Cyano is exclusively on the rocks. Looking under microscope the left side of tank is mostly cyano which hydrogen peroxide show impact, right side of tank is mostly spirolina which Chemiclean should impact. Hesitant to use Chemiclean as I'm afraid that's part of what drove things to this point since I used it previously. Tried H2O2 treatment for several weeks for the cyano but saw no impact.

I am thinking about a UV but it's cost prohibitive with 240 gallon system. At recommended 1 watt per 3 gallons thats an 80 watt unit which is pretty hefty price. I've also tried blackouts with no impact. I know it sounds like I've messed with things a lot but for the most part I've left things alone hoping it will right itself and that's why I've been fighting it for so long.

Any suggestions? I really don't want to tear things down.
That PO reading is pretty darn high. Are you using a Hanna ULR to get that reading? If so, I would recommend GRADUALLY knocking it back. Like over several months into .3 - .4 range.

I am gonna speculate that the cyano is coming from an imbalance between NO3 and PO4. Nitrates?
 

Mickey

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I am working on knocking the Phosphate level back slowly. But as an aside and I don't want to derail this thread, check out Richard Ross's posts (R2R name Thales) on his phosphate level which in the past has been similar to mine.

Anyway, Nitrates as of this morning were 23.3. So not too bad but could be lower. Again levels have been like this for many years and only fairly recently have I had problems.

I also took some time to check out a couple samples under the microscope today. It appears things might be improving even though things still look horrible. I saw some isolated examples of dinos of various types, definitely some LCA and Prorocentrum, Didn't find any Ostreopsis even though a month or so ago that's predominantly what I found. I didn't see any big conglomerations in the samples I pulled but I'll check again and try bigger samples in a few days. I also still saw occasional strands of both spirulina and cyano under the scope. The cyano too seems lessened.

My plan lately has been hanging filter material at night to capture what I can of any dinos in the water column, raised the temperature to about 82 and keeping the pH over 8.1 and preferable around 8.3. So for now I'll stay the course and keep syphoning what I can out.
 

badIuck

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What do you guys think - dinos? 3 years ago I had Prorocentrum dinos and they looked and moved a lot different. These things here are also more flailing around rather than scooting. Would greatly appreciate an ID :)

IMG-20240320-WA0019.jpg IMG-20240320-WA0021.jpg IMG-20240320-WA0031.jpg

edit: I think they might be Ostreopsis Dinos because of their "pointy heads"... also in videos of Ostreopsis they are also more flailing rather than scooting.
 
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ScottB

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What do you guys think - dinos? 3 years ago I had Prorocentrum dinos and they looked and moved a lot different. These things here are also more flailing around rather than scooting. Would greatly appreciate an ID :)

IMG-20240320-WA0019.jpg IMG-20240320-WA0021.jpg IMG-20240320-WA0031.jpg

edit: I think they might be Ostreopsis Dinos because of their "pointy heads"... also in videos of Ostreopsis they are also more flailing rather than scooting.
Yup, ostreopsis. A quality UV is your friend. 1 watt per 3 gallons of water volume. Do NOT go by the manufacturer's recommendation. Run a SLOW feed pump as these little buggers have some armor and need to be cooked thoroughly.
 

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Yup, ostreopsis. A quality UV is your friend. 1 watt per 3 gallons of water volume. Do NOT go by the manufacturer's recommendation. Run a SLOW feed pump as these little buggers have some armor and need to be cooked thoroughly.
Question on battling LCA...I have low nutrients as part of the cause. I am dosing NeoPhos and NeoNitrate. I can't seem to get my levels up to where I want them ...Phosphate=.1 and Nitrate=5-6.

How much phosphate can I add in a short period of time without causing a different problem, specifically an algae outbreak? For example. if my phosphate reading is .02, can I add enough Neophos to get to .1 in one dose? I have tried to do this over several days and it keeps dropping back to zero. I am wondering about dosing enough in one day to jumnp to .1 and then manage it daily from that level, but wondering what harm I would do.

Thanks
 

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Question on battling LCA...I have low nutrients as part of the cause. I am dosing NeoPhos and NeoNitrate. I can't seem to get my levels up to where I want them ...Phosphate=.1 and Nitrate=5-6.

How much phosphate can I add in a short period of time without causing a different problem, specifically an algae outbreak? For example. if my phosphate reading is .02, can I add enough Neophos to get to .1 in one dose? I have tried to do this over several days and it keeps dropping back to zero. I am wondering about dosing enough in one day to jumnp to .1 and then manage it daily from that level, but wondering what harm I would do.

Thanks
Maintaining PO4 in the water column can require a LOT of dosing because it gets bound to the rock and sand. Once your rock & sand is saturated with enough PO4, it will require little to no additional dosing. How much solution is required is therefore a trial and error process in my experience.

As to the potential to "overdose" PO4, I wouldn't sweat that too much. I run pretty high nutrients and there is no algae in either of my systems. I know many believe high nutrients is begging for nuisance algae, but in a MATURE system it really doesn't work that way. There is not a direct causal relationship.
 

badIuck

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I am also in the process of setting up a new tank with dry rock - hence the Ostreopsis issue - and I was dosing regular po4 solutions after the manufacturers recommendation for 4 weeks and couldn't get PO4 detectable. Then for 2 days I added 1 tablespoon of reef roids to my 160g total water volume per day and another 2 days later PO4 was 0,03 via Hanna checker.

I will get a 55w UV and let it run when the lights are off for some days. During the lighting period it won't do much anyways since they are then bound to the rocks guess...
 

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I've also posted this in the Dino ID thread, but thought adding it here could be helpful for me and maybe others


Hi I'm hoping for an ID on these dinos. I think they could be Amphidinium, except that I'm finding quite a few dead snails, which makes me think they're the more toxic type. Once I know the type, hopefully I can figure out how to treat them.

Thanks in advance!




@thedon986 has suggested large cell amphidinium or prorocentrum.

I know dinos generally come from low nutrients, which hasn't been my experience. I've been dealing with consistently high nitrate and phos over the last few months, which rose slowly to around 50ppm nitrate and 0.1+ phos. I've been carbon dosing (vinegar) to address it. Last night phos was 0.08 and nitrate was 25.1, so I've reduced the vinegar from 75ml to 60ml today (125g tank, and it took a long time to get the dosing high enough to reduce nitrate).

I've been finding dead snails which initially made me think ostreo but I'm definitely not convinced by the microscope images.
 

Buckerberg

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Seems like I am also part of the dino club.
6month old dry rock tank
Anyone that can confirm my ID as I think I spotted 2 species in my tank Ostreopsis & Large Cell Amphidinium





Would appreciate confirmation of my ID - Thx
 

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So question for the group. I have LCA as ID'd by my scope. Living in my sand bed. My tank is 3yrs old. Low Nutrients started the slide. I have done the following things in my battle:
1.) Increased Phosphate to .1 range.
2.) Increase Nitrate to 5-10ppm range
3) Weekly Dosing Silicate to keep level around 2PPM
4.) Dosing Phyto every other day
5.) Added Copepods
6.) Increased CUC including Cerith snails.

The other thing I am doing but not quite sure on how often and how much is that I does Microbacter7. I also have Dr. Tims product.

Because my problem started with low N and P, does the age of my systems (3yrs) matter when it comes to dosing bacteria?

How often and how much should I be dosing?
 

lmfbs

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So question for the group. I have LCA as ID'd by my scope. Living in my sand bed. My tank is 3yrs old. Low Nutrients started the slide. I have done the following things in my battle:
1.) Increased Phosphate to .1 range.
2.) Increase Nitrate to 5-10ppm range
3) Weekly Dosing Silicate to keep level around 2PPM
4.) Dosing Phyto every other day
5.) Added Copepods
6.) Increased CUC including Cerith snails.

The other thing I am doing but not quite sure on how often and how much is that I does Microbacter7. I also have Dr. Tims product.

Because my problem started with low N and P, does the age of my systems (3yrs) matter when it comes to dosing bacteria?

How often and how much should I be dosing?
What sort of silicate are you using and what are you using to test for it?
 
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