Cycling an Aquarium

vetteguy53081

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Update#3: Day 8. Still at ammonia 2.0ppm and everything else 0. Ph continues to bounce between 7.6 and 7.8. My temperature is right at 78 degrees, and salinity has remained constant at 1.025. I have not added any additional ammonium chloride, and have continued to dose the MB7, per the directions (figured I should follow the directions of 2 weeks to completion). I will continue checking all levels daily. But find it very odd that I have still yet to see any decline in ammonia levels. Even the dr. tims info says I should have seen levels decline at least somewhat by now.
What test kit are you using ?
 

vetteguy53081

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API for ammonia, nitrite, and nitrate. It has an april 2022 expiration date. And if i check ammonia against RODI water it shows 0.
There was a reason I asked. I figured with those zero readings it was API. API is notorious and known for false readings and has dissappointed many reefers hence the Low price for a master kit.
Youre likely getting false readings and values are higher than you realize. I would suggest taking a good water sample to a trusted LFS that does not use api kits for testing and see what results they come up with.
 

brandon429

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Blanking on non reef water has no application in reefing

These tests misrepresent reef water and known trace levels of nitrogen species tanks maintain.

All you have to do to meet the start date on your bottle bac is change water and begin on that date on the label. Do Tims is ten days


Change water is key...on the date the bottle bac label says.

If you had seneye none of this would be an issue.

Don't think test misreads only affect cyclers. Here's two pages of fully matured reefs doubting ammonia, mis testing.

And then we run a seneye...


Right now your tank is early stages agreed

Wait a bit longer but once it's locked it's locked, and your tester may not show it. Change water and begin. Seneye shows dosed reefs instantly able to handle ammonia, the other kits often don't. MB7 is the last bacteria I'd ever use for cycling.


Dr. Tims will work fine.
 
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DowntownJosh

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Blanking on non reef water has no application in reefing

These tests misrepresent reef water and known trace levels of nitrogen species tanks maintain.

All you have to do to meet the start date on your bottle bac is change water and begin on that date on the label. Do Tims is ten days


Change water is key...on the date the bottle bac label says.

If you had seneye none of this would be an issue.

Don't think test misreads only affect cyclers. Here's two pages of fully matured reefs doubting ammonia, mis testing.

And then we run a seneye...


Right now your tank is early stages agreed

Wait a bit longer but once it's locked it's locked, and your tester may not show it. Change water and begin. Seneye shows dosed reefs instantly able to handle ammonia, the other kits often don't. MB7 is the last bacteria I'd ever use for cycling.


Dr. Tims will work fine.
Thanks for the info. How much of a water change do you recommend, percentage wise?
 

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Cycling a new aquarium.

One of the earliest topics a new aquarium hobbyist needs to learn is how to properly cycle their aquarium. There is a ton of information on this process and many different methods on how to accomplish it. There are many different chemical and biological cycles our tank goes through as it matures but this addresses the one most commonly discussed.


What is cycling?

The term cycling comes from the process known as the “Nitrogen Cycle”. When a plant or animal decays, or an animal expels waste, nitrogen is released. In our aquariums we initially see this as ammonia. Bacteria converts the ammonia to nitrite and then to nitrate. When we have enough bacteria to quickly convert ammonia to nitrate, we say that our tank has cycled. This is deceiving because the process doesn’t stop at this point and it is never complete. For this reason we need to think of this as establishing a large enough bacteria population to support fish, not completing a cycle.
DSC_0048.JPG

Ammonia is a waste product of a fish’s cellular activity which is expelled through their gills. If the ammonia in the water is higher than in their blood it cannot be released and builds up in the fish causing cell damage. Nitrosomonas bacteria use ammonia as food and convert it to nitrite.

Nitrite is also harmful to fish. In a fish, nitrites hinder the ability of its blood to carry oxygen. Nitrites are a serious issue in fresh water systems. The same receptors in a fish that would absorb nitrites have a higher affinity for chlorides. The chlorides in saltwater block nitrites from being absorbed and protects the fish in marine systems. We rely on a different species of bacteria, Nitrobacter, to convert nitrite to nitrate.

Nitrates are relatively harmless for fish unless it reaches very high levels. Nitrates leaves our tank in any number of ways. We get rid of it via water changes. Algae and some corals can consume it as food.

The last part of the cycle is when nitrate is converted to nitrogen gas. This is done by anaerobic bacteria inside porous rock or within deep sand beds. Not every aquarium has the necessary conditions for this to occur

What conditions do I need for the cycle to occur?

To provide a good environment for the bacteria, we only need to make sure they are wet, oxygenated, and the pH is between 6.5 and 8.5. They will grow best at a temperature close to where we keep our tanks however they will still survive and reproduce at temperatures between 45F and 100F. We also want to monitor ammonia during this process. If ammonia gets over 5ppm it can slow down the rate bacteria reproduce. Above 10ppm it can stall the cycle.
frag tank (2).JPG

How does rock affect the cycle?

The rock in a marine tank is the core of its filtration system. We use porous rock to provide as much surface area as possible for bacteria to grow on. The water flowing around the rock brings the ammonia and nitrites to the bacteria. If the rock is large enough it may have anaerobic bacteria deep within it that can convert the nitrates to nitrogen gas.

There are many types of rock that are available in our hobby. I won’t get into all of the different types but I do want to address how dry and live rock impacts the cycle

Dry rock is just that. It is rock that has been dried out and has nothing alive on it. Dry rock can be “clean” where it is considered safe to put directly into an aquarium. If it is not clean it will need to be cured prior to use. Either way, it will not contain nitrifying bacteria. This isn’t a problem as nitrifying bacteria are everywhere and it would be impossible to keep them out of our aquarium even if we wanted to.

Live rock is rock that does have living bacteria on it. The main reason to use live rock is to speed up the cycling process. Just like with dry rock, not all live rock is ready to put immediately in an aquarium. For simplicity I am going to put live rock in 2 broad categories. If your live rock came directly out of a marine system and is kept submerged in quality water during transport you can put it directly into your tank. If the rock was exposed to air or shipped damp then it needs to be cured prior to use. The bacteria on this rock will be fine but other living things may have died and should be removed prior to putting it in your tank.
fish.jpg


How do I feed the bacteria?

There are many different takes on this and it is largely a matter of opinion. So here are the most common ways from worst to best in my opinion.

Adding a fish – while it will work as an ammonia source, why would you make a fish suffer in an environment in which it can’t properly shed its toxins?

Ghost feeding – When you add food to the tank you are adding much more than just what breaks down to ammonia. While none of it will be a problem, other than possibly algae, this is an uncontrolled process.

Adding a shrimp – The shrimp will decay and create ammonia, but again, this is an uncontrolled process. How much ammonia will this add and how quickly? I don’t know.

Dosing pure ammonia – This is the only method I will ever use in the future. You can measure exactly how much you need to add to achieve a specific level of ammonia. You can measure just how quickly your bacteria consume it to judge the health of your bacteria population.

How do I recommend doing it?

I’m glad you asked! I’ll start from the point where the tank is set up, filled, has flow, and temperature is in the normal range. It doesn’t matter if you used live or dry rock.

I will either use pure ammonia or ammonium chloride to raise the total ammonia to 2ppm. I will test for ammonia, nitrites, and nitrates daily until ammonia is near 0ppm. I will then dose it back up to 2ppm while continuing daily tests. I will repeat this process in a smaller tank until ammonia goes from 2ppm to 0ppm within 24 hours. In tanks that are 90g+ that will be stocked slowly I will only dose to 1ppm ammonia after the initial dose and consider it cycled when it drops from 1ppm to 0ppm in 24 hours. This is to limit nitrates in larger tanks. Keep in mind that a 120g system that drops 1ppm in a day can support more fish than a 40g tank that drops 2ppm in a day.

IMG_0527 (2).JPG

But I still have questions!


Ok, let me see if I can answer some of the more common ones.


If nitrifying bacteria are everywhere, why are “bacteria in a bottle” products so popular?

Nitrifying bacteria are everywhere so that isn’t the problem. Some bacteria can double their population in 20 minutes. Luck would have it that these tend to be the more poisonous kind. Nitrifying bacteria are relatively slow reproducers and it takes between 8 and 24 hours for them to double their population. If you start with dry rocks it can take a month or two to produce enough bacteria to support even a few small fish. Using bacteria in a bottle instantly provides a larger source of bacteria to speed up the front end of this process.

My cycle went fine for 3 weeks but now it stalled, what happened?

It could be any number of things. Did you let your ammonia go to high? Do you have a pH issue?

Your nitrifying bacteria may be being outcompeted for resources with other bacteria and/or algae. They may process ammonia fine at first but they will eventually go dormant. When this happens, your cycle will stall until a more marine friendly bacterium reproduces enough to begin the cycling process again. Bacteria in your tank are in constant competition for food. Eventually the one best suited to your specific system will thrive.


Can I vacuum out too much bacteria by cleaning? Will cleaning my sand bed cause my tank to cycle?

No, you cannot hurt your bacteria population by routine vacuuming or water changes. These bacteria are very difficult to remove from solid surfaces and would be difficult to remove even with scrubbing.

If your sand bed is very dirty you may cause an ammonia spike that is larger than your current bacteria population can handle but you haven’t done any harm to your bacteria population.

Can I use old tank water to cut back on my new tanks cycle time?

You can, but it won’t help much. The majority of the bacteria is going to be growing on hard surfaces, not floating in the water. There will be some, however, so you will likely introduce a strain of bacteria into your new tank that will eventually work very well.

I never measured any nitrite during my cycle, is this a problem?

No, odds are this is not a problem. It is possible that you had a larger initial population of Nitrobacter bacteria (nitrite eaters) compared to Nitrosomonas bacteria (ammonia eaters). In this case the nitrite was processed to nitrate almost as soon as it was produced.

Another possibility is that you have a large population of Nitrospira bacteria. These bacteria convert ammonia into nitrite and then to nitrate within the same cell structure so the nitrite is never released into the water to be measured.

My tank has been empty for 6 months, did my bacteria starve?

No. It is almost impossible to starve bacteria. They will adjust their metabolism and reproduction rates based on the food supply. As the amount of available food goes down, they will process it slower and reproduce less. If they go without food long enough they will go into a cystic stage (typically around a year without food). Once food is available again it will take longer for them to recover from this stage but they are still viable.

This does not mean that your tank is ready for fish after sitting dormant for up to a year. Bacteria is part of the food chain and is consumed by many different organisms. Just because your bacteria didn’t starve doesn’t mean it didn’t get eaten or is still viable. Every aquarium will respond differently based on its unique biology.

Why can I add more fish to an older aquarium faster than a new aquarium?

Let’s say you have 2 identical aquariums set up one year apart. They both have the exact same number and size of fish and both have 0 ppm ammonia and nitrite. They must have the same amount of bacteria, right? Well, no. The older tank will have a larger population of bacteria that is processing ammonia at a slower rate. The younger tank will have a smaller population of bacteria with faster metabolisms. If you add new fish to the younger tank you need to wait for the population to increase which can take days. In an older aquarium, the bacteria only need to become more active which can happen in a matter of hours.
fts11.jpg
Absolutely awesome!! I have read several guides to cycling and this is the most user friendly and simple enough to understand without being "Dummy material".

I am about to set up a 20 Gallon nano tank, and have had tanks in the past. The cycling was always a bit of hit and miss and now I know why. I have already ordered Dr Tim's Ammonia and One and Only, as reading these seem to have good reviews on most places.

I am hoping to achieve a nano reef with soft coral and maybe 3 or 4 small fish. so thanks again for your awesome guide.
 

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Josh the water change % doesn't matter much. its for a cleaner start, less algae fuel. not due to nh3 buildup
Gotcha, well i went with 25% water change. Then grabbed 2 clownfish from wwc yesterday.

Today my levels:

Ammonia: <0.15 (Salifert)
Nitrite/Nitrate: Near 0, tough to say with the color chart (API)
Phosphate: 0.16 (Hanna Meter)
Calcium: 414 (Hanna Meter)
Alkalinity: 7.6 (Hanna Meter)
pH: 7.9 (Milwaukee Meter)
Temperature: 77.7 (Inkbird)
Salinity: 1.026 (Hanna Meter)

Do those look OK? Should i do anything else, or just wait a week or 2 and add another fish?

Thanks again for all the help!
 

brandon429

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post an update pic of the tank

that shows us the working surface area and its contact position, the hallmarks of biofilter assessment.

once TAN conversion is applied to that ammonia reading above, that's not too far off what seneye would say. a thousandth of a ppm diff/pretty close above. What controls your fish addition wont be limited by nh3 ammonia control

it will be limited by ich, velvet, brook, neo, see the fish disease forum its daily/every hour.

quarantine and fallow are the top best methods for stopping that loss. other methods exist, just not in this forum:

adding fish isn't going to stress your cycle, the tank can handle what you have planned. if they're all previously qt fish, then add them based on behavior, how much you want their waste during uglies phase etc

all my builds in message are stacked in coral long before they're stacked in fish. we wait on fish, but not due to cycling limitations, because they're fertilizer pumps and during uglies phase its really hard to have many fish in the way of cleaning.
 
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post an update pic of the tank

that shows us the working surface area and its contact position, the hallmarks of biofilter assessment.
lights off and on pictures.

I'd like to add coral, but I read you need to wait until 6 to 8 weeks to add them, until levels are more stable. Should I add some starters sooner?

20210321_190340.jpg 20210320_170621.jpg
20210321_191002.jpg
20210321_191052.jpg
 
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brandon429

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Very nice setup, high surface area right in the middle of the contact zone looking sharp. Its ok to add or wait on corals either way is fine

we add starter corals and clean up crews now in my builds so we can make use of the system n get going a little, the retail feed options make up for lack of food web in the new system/ fancy feeds are available that work well for new corals. The BRS 4 month maturation approach is also ok too, either way np, nice reef
 

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Very nice setup, high surface area right in the middle of the contact zone looking sharp. Its ok to add or wait on corals either way is fine

we add starter corals and clean up crews now in my builds so we can make use of the system n get going a little, the retail feed options make up for lack of food web in the new system/ fancy feeds are available that work well for new corals. The BRS 4 month maturation approach is also ok too, either way np, nice reef
Awesome, thanks so much for all the help!!!!
 
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Awesome, thanks so much for all the help!!!!
Just keep in mind that the wait for adding coral is a completely separate issue than cycling the aquarium. There are equilibriums that happen in a reef tank, such as phosphate absorption/release by rock. You will also have algae and bacterial growth that will be constantly evolving and makes nutrient control very difficult in a new system.
If a new coral can handle the instability of a relatively new system you can go ahead and try it. If the coral is thought to be sensitive to swings then it is best to wait imo.
 

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Just keep in mind that the wait for adding coral is a completely separate issue than cycling the aquarium. There are equilibriums that happen in a reef tank, such as phosphate absorption/release by rock. You will also have algae and bacterial growth that will be constantly evolving and makes nutrient control very difficult in a new system.
If a new coral can handle the instability of a relatively new system you can go ahead and try it. If the coral is thought to be sensitive to swings then it is best to wait imo.
Great info! Any corals you would suggest to start with? I don't have any set ones I have to have.
 
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Great info! Any corals you would suggest to start with? I don't have any set ones I have to have.
I'm not sure what your end goals are, but the faster growing zoanthids tend to be pretty tough. GSP is an option. Leather corals aren't my thing, but they are a good starter coral.
I do recommend against adding anything into your tank you don't want there long term.
 

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Ok so I have another update and I'm curious everyone's thoughts.

I'm now about 3 weeks in after using Dr. Tims one and only. After 2 weeks, I did a 20-25% water change, and added 2 clowns. They seem happy and gobble up food. However, over the last 2 days I have gotten a large nitrite/nitrate spike. I've now had the clowns about a week.

Should I be worried or do anything other than water changes?

I'm running my skimmer and a BRS GFO/Carbon reactor.

Current levels:

Ammonia: <0.15 (Salifert)
Nitrite: 1.0 (API Test)
Nitrate: 40 (API Test) (Yesterday I measured 20, and today 40)
Phosphate: 0.11 (Hanna Meter)
Calcium: 389 (Hanna Meter)
Alkalinity: 7.2 (Hanna Meter)
pH: 7.8 (Milwaukee Meter)
Temperature: 78.1 (Inkbird)
Salinity: 1.026 (Hanna Meter)
 
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Ok so I have another update and I'm curious everyone's thoughts.

I'm now about 3 weeks in after using Dr. Tims one and only. After 2 weeks, I did a 20-25% water change, and added 2 clowns. They seem happy and gobble up food. However, over the last 2 days I have gotten a large nitrite/nitrate spike. I've now had the clowns about a week.

Should I be worried or do anything other than water changes?

I'm running my skimmer and a BRS GFO/Carbon reactor.

Current levels:

Ammonia: <0.15 (Salifert)
Nitrite: 1.0 (API Test)
Nitrate: 40 (API Test) (Yesterday I measured 20, and today 40)
Phosphate: 0.11 (Hanna Meter)
Calcium: 389 (Hanna Meter)
Alkalinity: 7.2 (Hanna Meter)
pH: 7.8 (Milwaukee Meter)
Temperature: 78.1 (Inkbird)
Salinity: 1.026 (Hanna Meter)
For some reason, Dr Tim's doesn't seem to process nitrite as well. Fortunately, nitrite is only a serious issue in fresh water systems.
Your nitrate is only indicating high because of the nitrite. Nitrate tests work by breaking nitrate down into nitrite and measuring it so they are only accurate when nitrite is 0.
Just continue to monitor it. I suspect you will see nitrite and nitrate both drop shortly before seeing nitrate start climbing again.
 

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For some reason, Dr Tim's doesn't seem to process nitrite as well. Fortunately, nitrite is only a serious issue in fresh water systems.
Your nitrate is only indicating high because of the nitrite. Nitrate tests work by breaking nitrate down into nitrite and measuring it so they are only accurate when nitrite is 0.
Just continue to monitor it. I suspect you will see nitrite and nitrate both drop shortly before seeing nitrate start climbing again.
ok, thanks for the info!
 

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I’m post 15+ days on my bottle bacterial start, and all of testing parameters are on point to where I’m going to do a 10% water change on 4/7. I’m not sure if I’m adding fish right away, or if I’m going to let the tank settle in some more. While iI’m in this limbo period, or if I decide to go with letting the tank sit without any inhabitants, do I still need to ghost feed, or just let the tank run without any additional feeding/supplements?
 
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I’m post 15+ days on my bottle bacterial start, and all of testing parameters are on point to where I’m going to do a 10% water change on 4/7. I’m not sure if I’m adding fish right away, or if I’m going to let the tank settle in some more. While iI’m in this limbo period, or if I decide to go with letting the tank sit without any inhabitants, do I still need to ghost feed, or just let the tank run without any additional feeding/supplements?
You can ghost feed if you want to increase the bacterial population further at the expense of building up nitrates. If you don't ghost feed, the aquarium will maintain its bacteria population for months. I don't feel there is a right or wrong answer, just personal preference.
 
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