65 gallon - multiple disease problems

Smo

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I have been reading thru this thread with great interest because I am dealing with a similar challenge. I thought I could get away with not quarantining in a new 65 gallon reef tank—my first! The DT had been running fine with my first two fish, an ocellaris and percula clowns. A month after the clowns I added a snowy lawnmower blenny, melanurus wrasse, and sailfin tang.

The blenny did great for two weeks, then one morning I noticed he was breathing very heavily. There were no other obvious symptoms. That evening I found him lying on his side. His color was bleached and the back half of his body towards his tail was a grayish color and his tail fin was disintegrating. He died within a couple of hours. None of the other fish displayed any symptoms including abnormal behavior. In retrospect—that should have been a warning signal.

Thinking the blenny was just an outlier—I added a royal gramma a couple of weeks later. After about a week I noticed white mucus patches starting to form on its body and its eyes began to get cloudy. The sailfin tang now displayed classic Ich white spots on its pectoral fins and body but otherwise behaved normally. It’s a great eater—too good. At this point I knew I needed to take action and set up a 20 gallon QT/hospital tank and began cycling. The two clowns also started to show signs of white mucus patches and their eyes began to cloud over. They along with the gramma lost much of their color. The gramma died before I could begin to treat it. I managed to catch the two clowns in the DT and performed fresh water baths. (They were nearly blind.) The ocellaris was too far gone and only lasted a few more hours. The percula appeared to make a miraculous recovery. The next morning it was nearly its old self again…even started eating. During this time all the spots disappeared on the sailfin tang. It never slowed down. There were times when I thought its breathing was just slightly above normal.

About five days later all the previous symptoms returned to the percula. I gave it another FW bath as I transferred it and the remaining melanurus wrasse, and sailfin tang to the QT. The next morning the clown looked great again. Not knowing for sure what I was dealing with (never saw any flukes during the FW dips but could easily have missed them in the clear container I used)—over the next four days I reduced specific gravity from 1.025 to 1.015 (to deal with potential flukes as well as improve oxygen), temperature from 78 deg to 73 deg. I was still waiting on medications.

In preparation for treating I read thru many of Humble Fish’s threads on disease diagnosis and treatment. I have ordered and received most of the “med cabinet” recommendations (except CP). I have Coppersafe, Cupramine and Cuprion as well as Seachem, Salifert and API Cu test kits. I wasn’t sure which might be best if I went the copper route with the melanurus wrasse. I am pretty convinced I have an Ich problem to deal with. Although, currently the Ich does not appear to be an immediate emergency. The clowns and gramma could have had flukes and they also may have Brook. I have thought long and hard about hypo salinity but am concerned about its effectiveness for all strains of Ich. So I have delayed starting a copper treatment just yet. All three fish appear normal, are eating and behaving reasonably normal considering they have been up routed from their home.

Yesterday, thinking I should deal with Brook if the clown has it, I performed a 90 minute acriflavine bath on all three fish using Ruby Reef Rally (Acriflavine-MS I ordered has not yet arrived). The fish have been in QT for 6 days now and showing no adverse symptoms of any kind. The percula is doing its bobble watching me as I type.

So, after the long winded diatribe above—what course of action should I perform next? Another acriflavine bath at the 3-4 day point. I have PraziPro available too but that treatment does not appear to be the highest priority right now. Given the fact the 20 gal QT is cycling reasonably well (I am performing daily ammonia, nitrite and nitrate tests—also have an ammonia alert badge and performing daily 12% water changes) I am reluctant to invest in another tank to perform the TTM. (I will do this for all future fish, however.)

Should I begin a copper treatment, if so, which one? I am concerned about the wrasse but leaning towards using the Cupramine since it appears to be a mainstay. I would slowly ramp it up to a therapeutic level of 0.5 ppm over 5-6 days, obviously observing the fish. Alternatively, given the risk to the wrasse—should I use the Coppersafe and accept the risk it may not be as effective? Once these little guys go back in the DT—I don’t want to have to remove the rocks again to fish them out should Ich return.

The QT has two HOB filters with foam only installed, an air powered internal foam filter and a small powerhead pointed towards the surface. There are no rocks, I have various PVC fittings, but I currently have a glass dish with sand for the wrasse to sleep in. He was less than enthusiastic the first couple nights without it. Should this come out if and when I begin a copper treatment?

Thanks in advance for any guidance, observations.
 

bif24701

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If you feel confidant of ich (crypto) I would continue to drop the Salinity to recommended hypo levels 1.008-1.009. Must use calibrated refractometer. I've had the best luck with that and I think I would have saved some fish if I had done that and not Cupramine. Flukes/black ich seems to be what I have currently and also the crypto but that is now gone. I only lost fish with hypo to jumping out.
 
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If you feel confidant of ich (crypto) I would continue to drop the Salinity to recommended hypo levels 1.008-1.009. Must use calibrated refractometer. I've had the best luck with that and I think I would have saved some fish if I had done that and not Cupramine. Flukes/black ich seems to be what I have currently and also the crypto but that is now gone. I only lost fish with hypo to jumping out.

Interesting. I have a cover on the tank. Also have two different refractometers and two different calibration fluids. Compared well to Milwaukee digital at LFS. My wife says I am OCD, but it is really CDO!
 

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@Smo It sounds like you are dealing with multiple disease problems. I would run all fish thru the full monty: copper + praziquantel + metronidazole.

You might even need a broad spectrum antibiotic (exs. Kanaplex, Furan-2) before this is all over.
 
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@Smo It sounds like you are dealing with multiple disease problems. I would run all fish thru the full monty: copper + praziquantel + metronidazole.

You might even need a broad spectrum antibiotic (exs. Kanaplex, Furan-2) before this is all over.

Thanks Humblefish! Treatment in that order? Which copper treatment would you suggest considering the wrasse? I understand the risk is all mine.

Some additional info...when the tang went into QT a week ago it had brownish spots on its face. They were not inflamed or raised--almost slight divots like scars. They have all since cleared up. I cannot attribute this to the reduced salinity or acriflavine bath two days ago.

The only symptoms I now observe are the clown appears a bit off. Respiration is normal, color may be a tad off, it tends to hover over one end of the 20 G long QT, and occasionally clamps its fins (have to look close to catch this). It is also only eating about half its normal amount, but not thinning yet.
 

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I would use Coppersafe and also pickup some API General Cure because that contains prazi + metro.

The brown might have been a secondary bacterial infection, but acriflavine being an antiseptic probably helped to clear it up.
 
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I would use Coppersafe and also pickup some API General Cure because that contains prazi + metro.

The brown might have been a secondary bacterial infection, but acriflavine being an antiseptic probably helped to clear it up.

Coppersafe for 30 days, ramping up slowly over next 5 days? Maintain salinity of 1.015 thru the 6th day and slowly raise to 1.019-ish or maintain where it is now? API General cure on the way.
 

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Coppersafe for 30 days, ramping up slowly over next 5 days? Maintain salinity of 1.015 thru the 6th day and slowly raise to 1.019-ish or maintain where it is now? API General cure on the way.

You got it. :)
 
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Here’s an update 48 hrs after beginning medication. The next day after the first API General Cure dose the clown appeared more active. At the 36 hour point it was eating better too. Below photo was taken yesterday (24 hour point) when there was better ambient light in the room. The tang has always been pretty active and today its respiratory rate may actually be lower than previously observed—especially when it was covered with white spots. I am hoping all this is not just wishful thinking. None of the fish appear to have any obvious signs of infection. All are active and eating.

IMG_0848.JPG


The second dose of General Cure just went into the tank. API says to do a 25% water change after 48 hours. Question…are two doses sufficient, would a third at the 96 hour point be beneficial? I have been running the specific gravity at 1.015 ppt for the past six days and plan to begin slowly raising it during the next partial water change.

I am ramping up the Coppersafe. I have 18 gallons in the system and have been adding 4.5 ml per day planning to get to the therapeutic level (1.5 – 2.0 ppm) by day 5. I am using an API copper test kit to measure the tank concentration before and after doses. I do have a small dish with sand for the wrasse, which does not appear to be impacting the copper concentration so far. He sleeps nearly 12 hours per day in it, which is consistent with his behavior in the DT.

Just performed a copper test a couple hours after the 3rd Coppersafe dose. It shows about 1.5 ppm (somewhere between the 1.0 and 2.0 color dots) assuming I am reading the results correctly. Look thru the vial from top to bottom on the white background, correct? It should only be at 1.0 ppm based on the amount of Coppersafe added so far (according to their directions). I did compensate for a two gallon water change I performed today (just prior to the 2nd General Cure dose) by adding an additional 1 ml of Coppersafe. I am inclined to believe repeatable test results and therefore will slow down the dosing until I am comfortable I know what I have for sure.

Ammonia has been essentially zero but the nitrites have crept up to 0.8 ppm. Zero would be ideal…how high can I tolerate nitrites in a QT? The Red Sea Marine Care Test Kit does not appear to be giving me any false positives in the presence of copper just yet. I also have a Seachem ammonia alert badge in the QT.
 

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Nitrites arn't something I even bother to test in QT. They can be bothersome, but with the frequent water changes until the tank cycles, it shouldn't reach high enough to bother the fish. Since I dno't bother to test the nitrites I can't tell you - for sure- how high they can tollerate, but I can say that as long as the Ammonia is zero you shouldn't have any problems.
 
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Nitrites arn't something I even bother to test in QT. They can be bothersome, but with the frequent water changes until the tank cycles, it shouldn't reach high enough to bother the fish. Since I dno't bother to test the nitrites I can't tell you - for sure- how high they can tollerate, but I can say that as long as the Ammonia is zero you shouldn't have any problems.
OK. Thanks. Will continue to test it because it is easy and I am curious how it will track.

Still surprised my copper is testing higher than expected.
 
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I am confused about the Coppersafe dosing. I have only added 9 ml of Coppersafe to my 18 gallon QT system. (I am using a plastic syringe to measure accurately.) I added an additional 1 ml for the two gallon water change I performed. The API Cu test kit indicates I am already at 1.5 ppm. How can this be when the recommended dosage is 5 ml/4 gal and for my system that would equate to 22.5 ml???

I did figure out I need to raise the test vial about ½” above the test card and then look thru it vertically to read accurately. I figured this out when testing the DT water as a control. The vial was only clear when raised off the paper, which I would expect for a reef tank with no copper in the water. Otherwise it is a tad darker if placed on the test card similar to Red Sea tests. For the QT now, this is a difference of 2.0 vs 1.5 ppm.

Second issue…the melanurus wrasse was out for a couple of hours this morning and acting very skittish, cowering around the PVC fittings on the bottom of the tank. He did eat some food, however. After that he has been in the sand bed all day and did not come out for an evening meal. I have to assume he does not like the copper treatment. He had been the only fish that has not displayed any adverse symptoms so far.

The percula clown and sailfin tang are doing great…out and about, very active and eating well. Their coloring has improved too. If the wrasse doesn’t show himself tomorrow I thing the best course of action may be to pull him out of the copper dosed QT and attempt a TTM using two other tanks, then keep him separate until the 30 day copper treatment is completed.

Thoughts anyone???
 
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Correction to above—I have added three doses of 4.5 ml for a total of 13.5 ml—not the 9 ml stated previously. I have also tested using a Salifert Cu test kit and it indicates slightly less than 1.0 ppm, which is where I would expect the concentration to be. So which do I believe, API or Salifert, or average the two?

I have not added a 4th dose this evening pending observation of the wrasse.
 

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Correction to above—I have added three doses of 4.5 ml for a total of 13.5 ml—not the 9 ml stated previously. I have also tested using a Salifert Cu test kit and it indicates slightly less than 1.0 ppm, which is where I would expect the concentration to be. So which do I believe, API or Salifert, or average the two?

I have not added a 4th dose this evening pending observation of the wrasse.

My experience with wrasses in copper (or any QT for that matter) is that they will hide in the sand for the first week or two. The fact that he came out at all and even ate some for you is a good thing. I would continue with the copper treatment. I would also believe the API kit for coppersafe above the other since the API kit is made to measure chelated copper and the other is made to measure ionic copper.
 
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Thanks Meredith! Out of conservatism, I held off on an additional Coppersafe dose last night. The wrasse was out this morning and he ate well along with the clown and tang. Not sure what was up with him yesterday. He had been doing well in the QT for the past 10 days.

I surpassed the 48 hour point following the 2nd dose of General Cure. As such, I performed a 25% water change (4.5 G) with 3.4 ml of Coppersafe added to compensate for the loss in the waste water. I am also slowly raising the salinity level back up to the 1.017-1.019 range. I have also added the 4th dose of Coppersafe. The API test kit shows I am at 2.0 ppm so I will hold off on the planned 5th dose and observe.

The 30 day clock just started! Only 62 more days of fallow on the DT. The pods are loving it.
 

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