Bacteria doesn't die off in any time frame we are concerned about. For the old school shrimp method, the shrimp needs to break down and rot which adds several days onto cycle times. Also rotting shrimp smells disgusting when you pull it out.Once upon a time .
the old school way to cycle was dose ammonia in any of many different ways .
Fishless was my favourite as it didn’t harm livestock .
I preferred using raw shrimp from the seafood counter .
drop
Them in and wait .
ammomia didn’t start that fast , but when it was detectable , it was there .
took a few days to transform to nitrite , which took a few more days to start detecting nitrates.
When nitrates were detected higher , with 0 nitrite and ammonia , the cycle was done .
I never had patience for daily dosing or remembering to dose every day .
That being said .
if the constant dose isn’t maintained at or above 2ppm .
I don’t think it would be effective or it would take longer to transform
if a dose is forgot , I remember reading something about the bacteria dying off .