Puffers DO NOT release a toxin when they die. Their flesh can be 10,000 stronger than a drop of cyanide. So as long as you or the inhabitants of the tank are not eating the flesh. There is no issue when a Puffer dies.Puffer fish died? That could trash your tank. They put out a toxin. Same with box fish. In 20 gal it would not take much to kill everything. 500 ppm nitrate is truly unlikely.