I used TBS LR for my Waterbox 20 cube. Great stuff and I continued to find beneficial hitch hikers well into the fourth month. I suspect that you will see some die off. I experienced that. The first to go was the macro algae and then you'll start losing some things you don't see. When this happened, I started to get some red turf algae so I added a large CUC. Don't make the same mistake I did; I added too many crew. SO I went through a second die off. Now everything seems to have equalized and I have such little algae in the tank that I never scrape the back wall so that the CUC has something to feast on when the rockwork is picked clean.