Ive never tried doing it with the female for establishment, I plan to do this with our next pairs to see how results differ. I have about a 70% success rate with pairing when the male is acclimated to the established female, but maybe it will yield better results vice versa?There is a bit of a debate on that, most agree that it should be the female in the box to establish the relationship but you can switch them when the new acclimation box gets there. Make sure to drill more holes in the box for water flow and try and add a lid if you can, either the lid of the tupperware (cut holes for light) or some eggcrate will work.