I am doing the TTM for preventing Ich and Velvet and dosing Prazi at day 2 and 8. I have personalized this protocol from information gather from Humble Fish and several R2R threads. I have read a lot of people advising to keep the fish in observation for 14 days after QT, but I am wondering if that is really necessary. I understand there is some risk of a bacterial infection developing later, but if I am preventing Ich and Velvet with the transfers and treating with Prazi, I am inclined to just put them in my DT at the end of QT if they are acting/eating normally. My protocol/timing is listed below with the basis for using this method for anyone who is unfamiliar.
Currently using TTM for 2x Benggai Cardinals in two 10 gallon tanks each with a heater, glass thermometer, 2" PVC tee, air stone (new each transfer), dedicated airline tubing, and a basic light. Each tank gets disinfected with vinegar after each use. Transferring them into a brand new, cycled, 65 Gallon DT + 20 gallon sump at the end.
Day 0 (Monday 2/15/21): Added to QT #1 6:20pm
Day 1 (Tuesday 2/16/21): Remain in QT #1.
Day 2 (Wednesday 2/17/21): 1st Transfer to QT #2 at 6:00am. Dose Praziquantel (1/2 tsp) at 6pm.
Day 3 (Thursday 2/18/21): 2nd Transfer to QT #1 at 6pm
Day 4 (Friday 2/19/21): Remain in QT #1.
Day 5 (Saturday 2/20/21): 3rd transfer to QT #2 at 6am.
Day 6 (Sunday 2/21/21): 4th transfer to QT #1 at 6pm
Day 7 (Monday 2/22/21): Remain in QT #1
Day 8 (Tuesday 2/23/21): Remain in QT #1. Dose Praziquantel (1/2 tsp) at 5pm.
Day 9 (Tuesday 2/24/21): 5th Transfer to QT #2 BEFORE 5:00pm
Day 10 (Wednesday 2/25/21): Remain in QT #2
Day 11 (Thursday 2/26/21): Remain in QT #2.
Day 12 (Friday 2/27/21): 6th Transfer to QT #1 BEFORE 4:00pm
Basis: Assuming worst case scenario, and velvet trophonts stop dropping off a fish immediately after going into a tank, you have around 48 hours until the tomonts begin releasing free swimmers (theronts) that reinfect the fish. So, a 36 hour transfer moves the fish away from the threat before this can happen. However, velvet trophonts can also remain on a fish for as long as 96 hours, so you have to do more than one transfer at 36 hours.
The "standard" 72 hour transfer (or 71 hours for good measure) can resume only near the tail end, once the threat of velvet is gone and you are just concerned about the possibility of Ich (which can remain on the fish for a longer duration - 7 days).
Day 0 - fish in tank
Day 1 - 1st transfer at 36 hours
Day 3 - 2nd transfer at 36 hours
Day 4 - 3rd transfer at 36 hours
Day 6 - 4th transfer at 36 hours
Day 9 - 5th transfer at 71 hours
Day 12 - 6th transfer at 71 hours
Currently using TTM for 2x Benggai Cardinals in two 10 gallon tanks each with a heater, glass thermometer, 2" PVC tee, air stone (new each transfer), dedicated airline tubing, and a basic light. Each tank gets disinfected with vinegar after each use. Transferring them into a brand new, cycled, 65 Gallon DT + 20 gallon sump at the end.
TTM to Eliminate Marine Ich (Cryptocaryon irritans) and Velvet
Day 0 (Monday 2/15/21): Added to QT #1 6:20pm
Day 1 (Tuesday 2/16/21): Remain in QT #1.
Day 2 (Wednesday 2/17/21): 1st Transfer to QT #2 at 6:00am. Dose Praziquantel (1/2 tsp) at 6pm.
Day 3 (Thursday 2/18/21): 2nd Transfer to QT #1 at 6pm
Day 4 (Friday 2/19/21): Remain in QT #1.
Day 5 (Saturday 2/20/21): 3rd transfer to QT #2 at 6am.
Day 6 (Sunday 2/21/21): 4th transfer to QT #1 at 6pm
Day 7 (Monday 2/22/21): Remain in QT #1
Day 8 (Tuesday 2/23/21): Remain in QT #1. Dose Praziquantel (1/2 tsp) at 5pm.
Day 9 (Tuesday 2/24/21): 5th Transfer to QT #2 BEFORE 5:00pm
Day 10 (Wednesday 2/25/21): Remain in QT #2
Day 11 (Thursday 2/26/21): Remain in QT #2.
Day 12 (Friday 2/27/21): 6th Transfer to QT #1 BEFORE 4:00pm
Basis: Assuming worst case scenario, and velvet trophonts stop dropping off a fish immediately after going into a tank, you have around 48 hours until the tomonts begin releasing free swimmers (theronts) that reinfect the fish. So, a 36 hour transfer moves the fish away from the threat before this can happen. However, velvet trophonts can also remain on a fish for as long as 96 hours, so you have to do more than one transfer at 36 hours.
The "standard" 72 hour transfer (or 71 hours for good measure) can resume only near the tail end, once the threat of velvet is gone and you are just concerned about the possibility of Ich (which can remain on the fish for a longer duration - 7 days).
Day 0 - fish in tank
Day 1 - 1st transfer at 36 hours
Day 3 - 2nd transfer at 36 hours
Day 4 - 3rd transfer at 36 hours
Day 6 - 4th transfer at 36 hours
Day 9 - 5th transfer at 71 hours
Day 12 - 6th transfer at 71 hours