I think it will be fine. Fish seem all good. I did this when I was deep cleaning my tank and doing a water change. The detritus is already settling. Also I siphoned out the back chambers for the very first time and I’ve had the tank for a few years lol and this was after I did a massive water change. I’m more so curious to see if anything bad happens but so far so good lol. I just stirred up my sand bed that’s all I did.