Tang Dead, Microscope Autopsy. Help ID.

[SaltwaterGuruNeeded]

Are there anything in these findings that would've killed my tang? He was breathing kinda fast starting yesterday or the day before...He died this morning. If you have suggestions I could go back and try to get a better picture of something.

@vetteguy53081

Let me know if I should take more pictures...I still have the fish to take more samples and still have the slides to look back at.

 

[DrZoidburg]

Pretty awesome of you to do this. Think I maybe see here gill fluke, and a ich cell(maybe). Other maybe pigment cells. To get any real cell damage you might need a little more mag, and cell stains.

 

[SaltwaterGuruNeeded]

Pretty awesome of you to do this. Think I maybe see here gill fluke, and a ich cell(maybe). Other maybe pigment cells. To get any real cell damage you might need a little more mag, and cell stains.

Could you point out which is the presumed gill fluke? And suspected ich cell?

 

[DrZoidburg]

Pic 1 100x gill fluke. Pic 2 looks a lot like an ich cell.

 

[DrZoidburg]

Again maybe.

 

[SaltwaterGuruNeeded]

Pic 1 100x gill fluke. Pic 2 looks a lot like an ich cell.

Thank you. It is so weird. I dosed prazipro in tank 3 times to still have a fluke.

 

[SaltwaterGuruNeeded]

Again maybe.

Yes, possibly velvet too?

 

[DrZoidburg]

velvet

 

[SaltwaterGuruNeeded]

velvet

Na, more like the first one,ich, it seems.

 

[DrZoidburg]

Some are resistant to prazi.

 

[SaltwaterGuruNeeded]

Some are resistant to prazi.

dang...

 

[DrZoidburg]

Looking at other thread though. Doesn't seem like a sever amount, could of been a combo of all things combined, and 02. Sorry didn't work out.

 

[SaltwaterGuruNeeded]

Looking at other thread though. Doesn't seem like a sever amount, could of been a combo of all things combined, and 02. Sorry didn't work out.

Yeah...If other fish(1 goby and 2 clowns) get sick or die, I will fallow my tank. Unless I can eradicate these diseases.

Edit: and yes probably was all them, but to me still a mystery, because I'm not seeing a whole lot of things, that fluke was the only one I saw on the gill sample.

Guessing it's a bacterial infection that killed him, but I still have no clue...

 

[SaltwaterGuruNeeded]

May have found why he died...

Looking at other thread though. Doesn't seem like a sever amount, could of been a combo of all things combined, and 02. Sorry didn't work out.

Are these worms?

 

[SaltwaterGuruNeeded]

Here is some more...

 

[SaltwaterGuruNeeded]

@Dr. Fish @Humblefish @vetteguy53081 @Jay Hemdal @ReefSquad Could I ask for some help identifying a good cause for my fish's death based on these pictures?

 

[SaltwaterGuruNeeded]

This is from the Gill squeeze. This is what came out. This is a video.

This one is the tangs Flesh.

 

[Jay Hemdal]

Here is some more...

Sorry - as I've said, MOTION is the big factor in evaluating disease issues under a microscope. Secondary to that is the magnification factor. You can see all sorts of things at high magnification that are simply artifacts. I remember once, 35 years ago, when I called my curator at the Shedd aquarium over to look at a slide from a dead fish, because I saw a huge amount of round cells and thought it was a disease - he said, well, Jay, what you have there are fish mucus cells.

The only thing I see that gives me pause for thought is the image titled, "tang flesh at 400x". That looks like a type of fluke. Trouble is, the magnification is all wrong - that issue would only look like that at around 80x power.

Microscopes are a great tool at lower power, looking for moving parasites. They fail though, at higher magnifications due to all of the artifacts that show up. The changeover takes place at around 180x. Anything higher than that really needs to be evaluated by tissue sent in for histopathology.

Jay

 

[SaltwaterGuruNeeded]

Ok, well thanks for the info. I have just attached 2 video's above your post. However videos don't always work on this site. I will try and find a way to use videos with more success in the future. But yes, I understand your point. Nothing really seemed to be alive to me, except at over about 600x and it's possible it's from what you've stated before about the effect of the things moving but they actually arent at higher power.

 

[Jay Hemdal]

This is from the Gill squeeze. This is what came out. This is a video.

This one is the tangs Flesh.

The vibrations of the tiny particles in these videos is the "Brownian motion" I mentioned. I also wonder sometimes if this motion isn't also caused by bacteria (too small to see) bumping into the large particles. In any event, I don't see any pathogens in these videos.

Jay