Salifert large bubble? Good or bad?

[alexanderthefishlover]

Hey,

I need help. I’m very confused here and can’t find a solid answer.

The salifert tests say to pull back the black suction part to 1ml not the liquid? But, sometimes the bubble is a different size??

Am I supposed to remove the red cap and suck back the reagent?? Or do I accept the bubble?

I’m so confused and I’m worried I’m doing this wrong.

PLEASE HELP ME

Please advise what phot (if any) are correct.

 

[alexanderthefishlover]

This link his looks like mine and he said don’t worry??

 

[PotatoPig]

The bubble is supposed to be there. It’s the air that’s in the nozzle when the plunger is fully depressed. At no point in the test will you be pushing air out so it won’t impact the results. Don’t worry about or try to displace it.

If you do displace it it means you’ve got some of the titration mixture left in the syringe and it may react, evaporate or degrade due to the open end, which will throw off your results.

All you are measuring in the test is the distance the plunger travels before the color changes. If the plunger moves from 1.0 to 0.19 then you used 0.81ml - look that value up on the chart for your result.

The bubble in front of the plunger doesn’t change the volume of liquid pushed out.

When doing the test set the black bit of the plunger to 1.0 as you have in the top pic, and then at the end of the test read the volume indicated by the same black bit of the plunger.

 

[alexanderthefishlover]

The bubble is supposed to be there. It’s the air that’s in the nozzle when the plunger is fully depressed. At no point in the test will you be pushing air out so it won’t impact the results. Don’t worry about or try to displace it.

If you do displace it it means you’ve got some of the titration mixture left in the syringe and it may react, evaporate or degrade due to the open end, which will throw off your results.

All you are measuring in the test is the distance the plunger travels before the color changes. If the plunger moves from 1.0 to 0.19 then you used 0.81ml - look that value up on the chart for your result.

The bubble in front of the plunger doesn’t change the volume of liquid pushed out.

Ok I have not been doing that. I just read what line it ends on?? I thought that’s how it worked?

That’s what the photos show? Ok I’m stupid I guess lol.

So basically it’s math. I need to see where the liquid is with the bubble, and then subtract the amount used and that’s the answer rather than the line it ends on?

 

[Cichlid Dad]

Hey,

I need help. I’m very confused here and can’t find a solid answer.

The salifert tests say to pull back the black suction part to 1ml not the liquid? But, sometimes the bubble is a different size??

Am I supposed to remove the red cap and suck back the reagent?? Or do I accept the bubble?

I’m so confused and I’m worried I’m doing this wrong.

PLEASE HELP ME

Please advise what phot (if any) are correct.

Push your pink tip further onto the syringe to lessen the bubble size. I draw in push all of the fluid out and draw again. When the plunger is at the 1 ml mark the bubble should be at 8.7- 9 ml mark

 

[PotatoPig]

Ok I have not been doing that. I just read what line it ends on?? I thought that’s how it worked?

That’s what the photos show? Ok I’m stupid I guess lol.

So basically it’s math. I need to see where the liquid is with the bubble, and then subtract the amount used and that’s the answer rather than the line it ends on?

From the photos below:
Start = 1.0
End = 0.2
Volume used = 0.8

The bubble in these tests does cause a lot of folks confusion, you’re definitely not the first.

 

[alexanderthefishlover]

The bubble is supposed to be there. It’s the air that’s in the nozzle when the plunger is fully depressed. At no point in the test will you be pushing air out so it won’t impact the results. Don’t worry about or try to displace it.

If you do displace it it means you’ve got some of the titration mixture left in the syringe and it may react, evaporate or degrade due to the open end, which will throw off your results.

All you are measuring in the test is the distance the plunger travels before the color changes. If the plunger moves from 1.0 to 0.19 then you used 0.81ml - look that value up on the chart for your result.

The bubble in front of the plunger doesn’t change the volume of liquid pushed out.

When doing the test set the black bit of the plunger to 1.0 as you have in the top pic, and then at the end of the test read the volume indicated by the same black bit of I

From the photos below:
Start = 1.0
End = 0.2
Volume used = 0.8

The bubble in these tests does cause a lot of folks confusion, you’re definitely not the first.

Omgsh I get it! Even if the air bubble is there you count it as if it was liquid ‍I get it thank you! I guess it doesn’t matter how much liquid is used. Just where ever the black plunger ends is the number to compare.

Thank you!

Ran my tests so different the results I was thinking my mg was through the roof I thought my calcium was too high I was reading where the liquid ended not the black plunger
Mom perfect results are much better now!

 

[tbrown]

Omgsh I get it! Even if the air bubble is there you count it as if it was liquid ‍I get it thank you! I guess it doesn’t matter how much liquid is used. Just where ever the black plunger ends is the number to compare.

Thank you!

Ran my tests so different the results I was thinking my mg was through the roof I thought my calcium was too high I was reading where the liquid ended not the black plunger
Mom perfect results are much better now!

It matters how much liquid is used. The main thing is, as shown in PotatoPig's photos, where you measure your start, use that same line to measure the end. So if you pull liquid to the 1.0 line, use the liquid line as your end level as well. The plunger is just easier to use since it's a solid black line. The air bubble is the amount of liquid in the pink tip, so that needs to be on when you're pulling liquid in and when you're titrating into the vial so don't remove the tip at all or run the entire test start to finish without the tip.

 

[tbrown]

That was kinda confusing I think.

You're measuring the amount of liquid used for titration.

The air bubble is equal to the amount of liquid in the pink tip.

When pulling liquid in AND when titrating, keep the pink tip on.

If you pull in liquid without the pink tip, keep the pink tip off for titration as well.

If you pull liquid to the 1.0, measure the end where the liquid is.

If you pull the plunger to 1.0, measure the end where the plunger is.

 

[alexanderthefishlover]

That was kinda confusing I think.

You're measuring the amount of liquid used for titration.

The air bubble is equal to the amount of liquid in the pink tip.

When pulling liquid in AND when titrating, keep the pink tip on.

If you pull in liquid without the pink tip, keep the pink tip off for titration as well.

If you pull liquid to the 1.0, measure the end where the liquid is.

If you pull the plunger to 1.0, measure the end where the plunger is.

I’m now more confused why does the amount of liquid matter? They don’t tell you to calculate the amount used?

So from the other guys photo his liquid was at 8 his black line was at 1.

It ended at 2 the black line so he’s going to go to the chart and find .20 correct?

Now I’m getting frustrated why can anyone make this clear

 

[alexanderthefishlover]

That was kinda confusing I think.

You're measuring the amount of liquid used for titration.

The air bubble is equal to the amount of liquid in the pink tip.

When pulling liquid in AND when titrating, keep the pink tip on.

If you pull in liquid without the pink tip, keep the pink tip off for titration as well.

If you pull liquid to the 1.0, measure the end where the liquid is.

If you pull the plunger to 1.0, measure the end where the plunger is.

As seen in instructions as shown by other commenter.

The air bubble was brought to the 1ml
Final reading .56ml (including air bubble)

Go to chart 6.7dKH.
So include the air bubble is what the instructions are saying also.

There is not need to know the amount used? There is no conversion from amount used to the chart?

So, once again. If the air bubble (black line plunger thing) is drawn to the 1ml (bubble + liquid) and liquid to the .8ml. And the black marker ends at 2ml. You’ll take that measurement (2ml) to the chart.

Yes or no? Anyone?

 

[tbrown]

I’m now more confused why does the amount of liquid matter? They don’t tell you to calculate the amount used?

So from the other guys photo his liquid was at 8 his black line was at 1.

It ended at 2 the black line so he’s going to go to the chart and find .20 correct?

Now I’m getting frustrated why can anyone make this clear

Sorry , you're right. Yes. That means he used 0.8 ml of liquid. Start at 1 ml, end at 0.2 ml equals 0.8 ml used. The results are based on what's left rather than what's used.

 

[tbrown]

I told you I was confusing in my answer.

 

[alexanderthefishlover]

From the photos below:
Start = 1.0
End = 0.2
Volume used = 0.8

The bubble in these tests does cause a lot of folks confusion, you’re definitely not the first.

Ok to clarify, I’d take that 2ml reading to the chart? Yes or no? Other commenters confused me once again

Volume used isn’t relevant is it? Because you ended at 2ml yet there is no liquid left in the syringe. But, youre still using that 2 ml (air bubble) as the result because you drew it to 1ml with the air bubble so you account for the air as if it was liquid?

 

[alexanderthefishlover]

Sorry , you're right. Yes. That means he used 0.8 ml of liquid. Start at 1 ml, end at 0.2 ml equals 0.8 ml used. The results are based on what's left rather than what's used.

Ok, so he’s going to use the 2ml and take that to the chart even if it’s just air left? We are assuming it’s liquid?

 

[tbrown]

Ok to clarify, I’d take that 2ml reading to the chart? Yes or no? Other commenters confused me once again

Volume used isn’t relevant is it? Because you ended at 2ml yet there is no liquid left in the syringe. But, youre still using that 2 ml (air bubble) as the result because you drew it to 1ml with the air bubble so you account for the air as if it was liquid?

Yes

 

[tbrown]

Ok, so he’s going to use the 2ml and take that to the chart even if it’s just air left? We are assuming it’s liquid?

Yes.

 

[alexanderthefishlover]

Yes.

Got it my tests are perfect now. I thought my numbers were so high

 

[tbrown]

The main points to take away from my confusing post: make sure if you pull liquid in without the pink tip on (as it looks like you may have done in one of your photos) run the test without the pink tip.

 

[alexanderthefishlover]

The main points to take away from my confusing post: make sure if you pull liquid in without the pink tip on (as it looks like you may have done in one of your photos) run the test without the pink tip.

100% I use the pink tip just wanted to show options.