I've decided to give phyto culture a go and am in the process of preparing the supplies.
Before I get started, I wanted to learn as much as possible about the procedures and be prepared so I've been reading and watching youtube videos but I still have some questions (I'm sure there will be more to come) and hope to hear from those who have the experience.
1. Many people seem to harvest and/or split a culture by pouring some of it into another container and continuing the existing culture by refilling freshly mixed saltwater and adding F/2 medium. When you pour it to harvest, won't it have some phyto getting stuck to the top side and mouth of the bottle? Could this become a problem (decay, contaminate, etc, being out of water and all) or is this not really an issue? Are you better off using a new sterilized bottle after each harvest? Or am I just getting paranoid and overthinking this?
2. For those who are using typical plastic bottles with narrow openings, how do you sterilize the bottle? Do you put hydrogen peroxide, bleach, or ethanol solution and then shake it? If you are using hydrogen peroxide or bleach solution, do you have to rinse it with water afterward or can you just pour it out and air dry? If you have to rinse it, won't there be a risk of contamination from the water you rinse it with?
3. Do you add F/2 after each harvest or during culture as well?
I will be back with more if I can think of any. Thank you in advance
Before I get started, I wanted to learn as much as possible about the procedures and be prepared so I've been reading and watching youtube videos but I still have some questions (I'm sure there will be more to come) and hope to hear from those who have the experience.
1. Many people seem to harvest and/or split a culture by pouring some of it into another container and continuing the existing culture by refilling freshly mixed saltwater and adding F/2 medium. When you pour it to harvest, won't it have some phyto getting stuck to the top side and mouth of the bottle? Could this become a problem (decay, contaminate, etc, being out of water and all) or is this not really an issue? Are you better off using a new sterilized bottle after each harvest? Or am I just getting paranoid and overthinking this?
2. For those who are using typical plastic bottles with narrow openings, how do you sterilize the bottle? Do you put hydrogen peroxide, bleach, or ethanol solution and then shake it? If you are using hydrogen peroxide or bleach solution, do you have to rinse it with water afterward or can you just pour it out and air dry? If you have to rinse it, won't there be a risk of contamination from the water you rinse it with?
3. Do you add F/2 after each harvest or during culture as well?
I will be back with more if I can think of any. Thank you in advance