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If you are tossing handfuls of food into the tank daily and still getting no ammonia readings, you can probably add quite a few fish at once. Your biological filter (nitrifying bacteria population) will grow (and shrink) based on the amount of ammonia added or produced in the tank. If you have an established 220 gallon with 12 fish then adding another 5 or 6 at once should be ok, but if your system is new, then you need to go slow in order for the bacterial populations to catch up.Is there a way to test how many fish your tank can handle at once?